Race-specific association between DNA methylation and body mass index: the Bogalusa Heart Study. D. Sun1,2, X. Fu3, S. Li1, Q. Li4, T. Chen4, Y. Lian4, C. Fernandez1, W. Chen1 1) Department of Epidemiology, School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA, USA; 2) Department of Epidemiology, School of Public Health, Peking University Health Science Center, Beijing, China; 3) Department of Biostatistics and Bioinformatics, School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA, USA; 4) Microarray Core Facility, University of Texas Southwestern Medical Center, Dallas, TX, USA.
This study test the hypothesis that genome-wide DNA methylation is associated with adiposity measured as body mass index (BMI). As part of the Bogalusa Heart Study, 968 adults aged 28-51 years were recruited between 2006 and 2010. Of these individuals, 830 participants (584 whites, 246 blacks) were examined only once and used as the discovery cohort; 138 participants (95 whites, 43 blacks) were examined twice 3.2 years apart and used as the replication cohort. The discovery findings were replicated by the baseline and follow-up sample. Genome-wide methylation of peripheral leukocyte DNA samples was measured by HumanMethylation450 BeadChip and sample and probe quality analysis was performed. In the association analyses by race, adjusting for age, gender and smoking, DNA methylation levels at 17 sites in whites and at 4 sites in blacks were found to be significantly associated with BMI in discovery cohort. As shown in the table below, 2 (cg06500161 and cg00574958) of the 17 sites in whites and 1 (cg26403843) of 4 sites in blacks were significant in both baseline and follow-up replication cohorts. In conclusion, DNA methylation changes in lipid metabolism-related genes are associated with BMI values, which provides potential race-specific epigenetic mechanisms underlying the development of obesity in whites and blacks.
Effect sizes and Bonferroni-adjusted P values
|CpG||Gene||Discovery cohort||Baseline replication||Follow-up replication|
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