New insights on human de novo mutation rate and parental age. W. S. W. Wong, B. Solomon, D. Bodian, D. Thach, R. Iyer, J. Vockley, J. Niederhuber Inova Translational Medicine Institute, Falls Church, VA.
Germline mutations have a major role to play in evolution. Much attention has been given to studying the pattern and rate of human mutations using biochemical or phylogenetic methods based on closely related species. Massively parallel sequencing technologies have given scientists the opportunity to study directly measured de novo mutations (DNMs) at an unprecedented scale. Here we report the largest study (to our knowledge) of de novo point mutations in humans, in which we used whole genome deep sequencing (~60x) data from 605 family trios (father, mother and newborn). These trios represent the first group of approximately 2,700 trios who have undergone whole-genome sequencing (WGS) through our pediatric-based WGS research studies. The fathers ages range from 17 to 63 years and the mothers ages range from 17 to 43 years. We identified over 23000 DNMs (~40 per newborn) in the autosomal chromosomes using a customized pipeline and infer that the mutation rate per basepair is around 1.2x10-8 per generation, well within the reported range in previous studies. We were also able to confirm that the total number of DNMs in the newborn was directly proportional to the paternal age (P <2x10-16). Maternal age is shown to have a small but significant positive effect on the number of DNMs passed onto the offspring, (P =0.003) , even after accounting for the paternal age. This contradicts the prior dogma that maternal age only has an effect on chromosomal abnormalities related to nondisjunction events. Furthermore, 5% (22 total) of newborns in the analyzed group were conceived with assisted reproductive technologies (ARTs), and these infants have on average 5 more DNMs (Bias corrected and accelerated bootstrap 95% Confidence Interval, 1.24 to 8.00) than those conceived naturally, after controlling for both parents ages. Both parents ages remain significant as independently correlated with DNMs even after the families that used ARTs were removed from the analysis. Our study enhances current knowledge related to the human germline mutational rates.
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