Mitochondrial Genome Database for Saudi Community. I. Alabdulkareem1, M. Albalwi1,2, M. Alharbi1, A. Alghamdi1, B. Alhamad1, M. Aljumah1 1) KAIMRC, National Guard Health Affairs, Riyadh, Riyadh, Saudi Arabia; 2) King Abdulaziz Medical City for National Guard, Pathology Department, Riyadh, Saudi Arabia.
This study is aiming to create an electronic database for all Non Mendelian inheritance among Saudi populations that is will be available for all researchers and health providers at the country. Phase one of this investigations is focusing on the establishment of the mitochondria genome sequencing in healthy (mtDNA). mtDNA as extracellular components that do not subdue to Mendelian inheritance mode that is genetically considered as complete different in terms of evolutionary origin since many of the evaluation scientists thought that mtDNA may be derivative from the circular genomes of the bacteria that were engulfed by the early ancestors (endosymbiotic hypothesis). mtDNA was sequenced using the solid 5500 and validate by Ion Personal Genome Machine [PGM] System. Studies showed that the Arab assembled mitochondrial genome contains 16,570 bp as compared to GenBank reference genome with 16,571 bp. Further analysis in mitochondrial genome detected 46 differences, with almost single point mutations and only a deletion of length one. Homology studies on 3,132 known mitochondrial sequences from NCBI with sequence length between 16,000 and 17,000 bp revealed more than 90% matches sequence to accession number EF184636 sequence. Reported single nucleotide polymorphisms (SNPs) are rs3927813 rs3928312 rs1599988 rs3021088 rs3929989, rs1978028 rs121434446, rs1981459, rs3902407, rs3915952, rs3926883, rs3899498 rs2835780, rs3135031, rs34799580. More subjects from healthy and diseased will be included in this investigation that will facilitate more understanding of many unexplained chronic diseases among the populations.
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